Research Article
Rhizosphere Streptomycetes Producing Antifungal to Candida albicans ATCC 10231 and C. albicans ATCC 24433 from Various Plants
@INPROCEEDINGS{10.4108/eai.2-5-2019.2284679, author={Eti Sumiati and Dewi Nur Anggraeni}, title={Rhizosphere Streptomycetes Producing Antifungal to Candida albicans ATCC 10231 and C. albicans ATCC 24433 from Various Plants}, proceedings={1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia}, publisher={EAI}, proceedings_a={ICOST}, year={2019}, month={6}, keywords={streptomycetes rhizosphere antibiotic antifungal}, doi={10.4108/eai.2-5-2019.2284679} }- Eti Sumiati
Dewi Nur Anggraeni
Year: 2019
Rhizosphere Streptomycetes Producing Antifungal to Candida albicans ATCC 10231 and C. albicans ATCC 24433 from Various Plants
ICOST
EAI
DOI: 10.4108/eai.2-5-2019.2284679
Abstract
A research on potency of rhizosphere streptomycetes producing antifungal on various plants (Cyperus rotundus L., Zea mays L., and Rhizopora mucronanata) was conducted. Twelve isolates of streptomycetes was tested to assess their potency in producing antifungal compound by using agar block method with Candida albicans ATCC 10231 and C. albicans ATCC 24433 as test fungi. The result showed that 3 isolates among 12 isolates showed their potency to produce antifungal. Based on their antifungal activity, 1 isolates (J10) were chosen to produce antifungal compound using fermentation method in GYE broth. Characterization of antifungal compound was carried out by using TLC, GC-MS and bioautographic test. The result of bioautography test showed that the spot from TLC of J10 isolates could inhibit C. albicans ATCC 10231 growth. Identification of antifungal compound of J10 by using TLC analysis and spray reagent suggested that then alkaloid, flavonoid, and phenol class of compound.