Research Article
The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV
@INPROCEEDINGS{10.4108/eai.13-11-2018.2283792, author={Rizki Rahmadian and Menkher Manjas and Roza Silvia and Hirowati Ali}, title={The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV}, proceedings={Proceedings of the 1st EAI International Conference on Medical And Health Research, ICoMHER November 13-14th 2018, Padang, West Sumatera, Indonesia}, publisher={EAI}, proceedings_a={ICOMHER}, year={2019}, month={5}, keywords={il-6 il-1β mesenchymal stem cells osteoarthritis}, doi={10.4108/eai.13-11-2018.2283792} }- Rizki Rahmadian
Menkher Manjas
Roza Silvia
Hirowati Ali
Year: 2019
The Effect of Mesenchymal Stem Cells on IL-1β and IL-6 in Synoviocytes-Derived Osteoarthritis Grade IV
ICOMHER
EAI
DOI: 10.4108/eai.13-11-2018.2283792
Abstract
Osteoarthritis is a degenerative joint disease involving activated synovial tissue due to inflammation. Cytokines seem to enhance the matrix components of lead to destruction of cartilage. Mesenchymal stem cells (MSCs) are widely used for cell-based nowadays approaches, thus activated cytokines, IL-1β andIL-6 as a result of synovial inflammation that are left to be investigated.This is an in vitro study of IV-IL and IL-6 on IV synoviocytes-derived osteoarthritis treated with MSCs. Cells were isolated using explants culture method and cultured in DMEM with 10% fetal bovine serum, 1% penicillin streptomycin, and amphotericin B. Cells were incubated at 370C and 5% CO2. After third passages, cells were co-cultured with MSCs. Expression of IL-1β and IL-6 are observed using realtime polymerase chain reaction (PCR) after 24 hours and 48 hours. The expression of IL-1β is marked up after 24 hours of co-cultured with MSCs (57.72 ± 18.37) compared to control and shows a tendency to downgrade after 48 hours of event, statictically not significant. However, IL-6 showed a higher expression in synoviocytes after 24 hours of co-cultured with MSCs, and was significantly down regulated after 48 hours. Both IL-1 β and IL-6 showed higher expressions on 24 hours co-culturing synoviocytes with MSCs. Not IL-1β, expression of IL-6 is significantly decreased after 48 hours MSC treatment, implying that MSV grade IV syndicarthritis-derived osteoarthritis.