Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey

Research Article

Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks

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  • @INPROCEEDINGS{10.4108/eai.7-9-2021.2315380,
        author={Asaad Faraj Hamzah and Wijdan Hussein Al-Tamimi},
        title={Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures  zones  of oil reservoir rocks},
        proceedings={Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey},
        publisher={EAI},
        proceedings_a={IMDC-IST},
        year={2022},
        month={1},
        keywords={biopolymer byproducts meor petroleum microbiology},
        doi={10.4108/eai.7-9-2021.2315380}
    }
    
  • Asaad Faraj Hamzah
    Wijdan Hussein Al-Tamimi
    Year: 2022
    Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks
    IMDC-IST
    EAI
    DOI: 10.4108/eai.7-9-2021.2315380
Asaad Faraj Hamzah1,*, Wijdan Hussein Al-Tamimi2
  • 1: Technical institute of Basrah , Southern Technical University, Basrah, Iraq
  • 2: Departments of Biology, Science Collage, University of Basrah, Basrah, Iraq
*Contact email: asaadfaraj@yahoo.com

Abstract

In this study, 21 environmental bacterial isolates isolated from different environmental sources in Basra Governorate identified phenotypically and genetically based on 16 rRNA gene sequence analysis were screened to reveal their ability to produce biopolymer based on the viscosity scale formed in the cultural media and use of Sudan Black stain. Six isolates showed their ability to produce biopolymer, and the two isolates of Bacillus licheniformis strain CC91, Bacillus thuringiensis strain LU3 recorded the best results. The isolate of the Bacillus licheniformis strain CC91 was tested in the process of sealing fractures and openings with high permeability for samples of oil reservoir rocks.