Research Article
Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources
@INPROCEEDINGS{10.4108/eai.7-9-2021.2315374, author={Mohammed Hussein Amer and Hasan Abd Ali Khudhair and Khwam R Hussein}, title={Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources}, proceedings={Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey}, publisher={EAI}, proceedings_a={IMDC-IST}, year={2022}, month={1}, keywords={klebsiella pneumoniae antibiotics resistance k1 and k2 genes}, doi={10.4108/eai.7-9-2021.2315374} }- Mohammed Hussein Amer
Hasan Abd Ali Khudhair
Khwam R Hussein
Year: 2022
Virulence Characteristics and Antibiotic Resistance of Klebsiella pneumoniae Isolated from Clinical Sources
IMDC-IST
EAI
DOI: 10.4108/eai.7-9-2021.2315374
Abstract
Klebsiella pneumoniae (K. pneumoniae) is a Gram-negative capsulated bacterium, one of the most relevant opportunistic pathogens, and causes various human infections. This study was aimed to determine the prevalence and antibiotics susceptibility pattern of K. pneumoniae that isolated from various clinical samples and to detect some virulence factors of it via molecular method. One hundred and eighty patients suffering from respiratory, urinary, wounds and burns infections were enrolled in this study and evaluated for the presence of K. pneumoniae infection via culturing, microscopical, motility and biochemical characteristics as well as API 20E and VITEK 2 systems. Molecular technology was used for detection the presence of K1 and K2 genes as well as the presence and sequencing 16S ribosomal ribonucleic acid (16S rRNA) gene. The results showed that a low prevalence of K. pneumoniae (27.8%) in Iraqi patients with higher positivity rate in sputum samples (42%) compared to other clinical samples.. According to the results of phylogenetic tree construction of 16S rRNA gene, we identified six mutated clinical isolates of K. pneumoniae that were deposited in GenBank under the accession numbers of MW642198, MW642199, MW642200, MW642201, MW642202 and MW642203. The mutated 16S rRNA gene stains exhibited a significantly higher resistance to all antibiotics types that used in this study.