Research Article
Investigating the Gene Expression Profiles of Immune Checkpoints in Breast Cancer Microenvironment and Peripheral Blood: A Comparative Study
@INPROCEEDINGS{10.4108/eai.7-9-2021.2314941, author={Howraa J Bakal and Khairallah A.S Mohammed and Naael H. Ali}, title={Investigating the Gene Expression Profiles of Immune Checkpoints in Breast Cancer Microenvironment and Peripheral Blood: A Comparative Study}, proceedings={Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey}, publisher={EAI}, proceedings_a={IMDC-IST}, year={2022}, month={1}, keywords={checkpoints gene expression ctla-4 foxp3}, doi={10.4108/eai.7-9-2021.2314941} }- Howraa J Bakal
Khairallah A.S Mohammed
Naael H. Ali
Year: 2022
Investigating the Gene Expression Profiles of Immune Checkpoints in Breast Cancer Microenvironment and Peripheral Blood: A Comparative Study
IMDC-IST
EAI
DOI: 10.4108/eai.7-9-2021.2314941
Abstract
This study aimed to evaluate and compare the expression of the immune checkpoints (FOXP3 and CTLA-4) in the tumour microenvironment and peripheral blood in patients with breast cancer. After total RNA extraction from blood and tissues from 36 women with breast cancer and 15 with breast benign tumours, the expression of the CTLA-4 and FOXP3 transcripts was assessed using real time-PCR. In breast cancer, the amounts of the CTLA-4 expression in the tissues were significantly higher than in blood (P<0.02). The expression of CTLA-4 in blood from patients with breast cancer was significantly lower compared with blood from the benign group (P<0.001). In a breast benign tumour, there was no difference in the expression of CTLA-4 mRNA in tissues and blood. The expression of FOXP3 in tissue from breast cancer was higher than the expression in blood, while it was similar in both tissues and blood from breast benign tumours.