Molecular Characterization of Staphylococcus Haemolyticus Isolated from Vaginitis and Some of Their Virulence Factors

Enas Alwaily; Ruaa Khazaal; Mohammed Flaih; Khwam Hussein

Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey

Research Article

Molecular Characterization of Staphylococcus Haemolyticus Isolated from Vaginitis and Some of Their Virulence Factors

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@INPROCEEDINGS{10.4108/eai.7-9-2021.2314816,
    author={Enas R. Alwaily and Ruaa M. Khazaal and Mohammed H. Flaih and Khwam R. Hussein},
    title={Molecular Characterization of Staphylococcus Haemolyticus Isolated from Vaginitis and Some of Their Virulence Factors},
    proceedings={Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey},
    publisher={EAI},
    proceedings_a={IMDC-IST},
    year={2022},
    month={1},
    keywords={16s rrna s haemolyticus antimicrobial susceptibility resistance genes virulence genes},
    doi={10.4108/eai.7-9-2021.2314816}
}

Enas R. Alwaily
Ruaa M. Khazaal
Mohammed H. Flaih
Khwam R. Hussein
Year: 2022
Molecular Characterization of Staphylococcus Haemolyticus Isolated from Vaginitis and Some of Their Virulence Factors
IMDC-IST
EAI
DOI: 10.4108/eai.7-9-2021.2314816

Enas R. Alwaily¹^,*, Ruaa M. Khazaal², Mohammed H. Flaih², Khwam R. Hussein³

1: College of Pharmacy, Al-Ayen University, Al-Nasiriyah, 64001, Iraq
2: Department of Medical Laboratory Techniques, Al-Nasiryah Technical Institute, Southern Technical University, Al-Nasiriyah, 64001, Iraq
3: Department of Nursing, Al-Nasiryah Technical Institute, Southern Technical University, Nasiriyah, 64001, Iraq

*Contact email: Enas.Kazem@alayen.edu.iq

Abstract

Vaginitis is still considered to be a global health problem for females. It is mainly occurred by bacterial vaginosis, trichomonal, candidal, and gonococcal vaginal infections. This study is aimed identify Staphylococcus haemolyticus in females infected with vaginitis and determine some of their virulence genes. A total of 250 vaginal swabs were collected from females with vaginitis. Of the 250 vaginal swabs, 40 isolates were showed bacterial growth of S. haemolyticus. In susceptibility testing, S. haemolyticus isolates were sensitive to some antibiotics, whereas other isolates were resistant. The amplified 16S rRNA gene was in all isolates, using conventional PCR, icaA, blaz, mecA, and SodA genes were detected in 30, 23, 32, and 24 samples, respectively. The sequencing analysis of all local isolates appeared slight mismatching in their sequencing. The PCR products of virulence factors of S. haemolyticus showed slight variations. This may explain different clinical manifestations and the infection severity.

Keywords: 16s rrna s haemolyticus antimicrobial susceptibility resistance genes virulence genes

Published: 2022-01-26
Publisher: EAI

: http://dx.doi.org/10.4108/eai.7-9-2021.2314816