Research Article
Metabolomic Analysis of Dendrophthoe pentandra (L.) Miq. Leaves via UPLC-QToF-MS Coupled with Multivariate Data Analysis using PCA
@INPROCEEDINGS{10.4108/eai.2-10-2018.2295577, author={Roihatul Mutiah and Atina Yuliandari and Rahmi Annisa and Burhan Ma’arif}, title={Metabolomic Analysis of Dendrophthoe pentandra (L.) Miq. Leaves via UPLC-QToF-MS Coupled with Multivariate Data Analysis using PCA}, proceedings={Proceedings of the 2nd International Conference on Quran and Hadith Studies Information Technology and Media in Conjunction with the 1st International Conference on Islam, Science and Technology, ICONQUHAS \& ICONIST, Bandung, October 2-4, 2018, Indonesia}, publisher={EAI}, proceedings_a={ICONQUHAS}, year={2020}, month={5}, keywords={metabolomics; dendrophthoe pentandra (l) miq; uplc-qtof-ms/ms; pca (pricipal component analysis)}, doi={10.4108/eai.2-10-2018.2295577} }- Roihatul Mutiah
Atina Yuliandari
Rahmi Annisa
Burhan Ma’arif
Year: 2020
Metabolomic Analysis of Dendrophthoe pentandra (L.) Miq. Leaves via UPLC-QToF-MS Coupled with Multivariate Data Analysis using PCA
ICONQUHAS
EAI
DOI: 10.4108/eai.2-10-2018.2295577
Abstract
Mango mistletoe (Dendrophthoe pentandra) is a semi-parasitic plant on a host that is potential as a medicinal plant. Thus, it is necessary to conduct metabolite profiling to determine the compounds contained in mango mistletoe leaves. This research aims to find out the difference of metabolite profile of mango mistletoe leaves obtained from Kediri, East Java; Pekalongan, Central Java; Batin Baru Mountain, Lampung and Tanjung, Selor Hilir in North Kalimantan. Extraction method with ultrasonic aid using 96% ethanol solvent with the ratio of 1:10 [b/v] was used to obtain condensed extract of mango mistletoe leaves. The four extracts from different regions were analyzed using UPLC-QToF-MS/MS, mobile phase of mixture of water/formic acid of 99.9/0.1 [v/v] and acetonitrile/formic acid of 99.9 / 0.1 [v/v] with a gradient elution system and a C18 stationary phase. The results of the analysis were then followed up by a chemometric analysis using PCA employing Minitab 17 software. There are differences in the metabolite among the four regions of extract acquisition. The grouping is based on similar metabolite which occurred in Kediri, East Java and Tanjung Selor Hilir, North Kalimantan. Allegedly, methyldioctylamine; scortechinone F; 3-Cyclohexyl-N- (Ethoxycarbonyl)-L-Alanyl-N-[(4S, 5E, 7R)-7-carbamoyl-9-methyl-5-decen-4-yl]-lysinamide; and Pheophorbide A were biomarker compounds based on the geographic origin.