Bioactivity of Pure Antibacterial Compounds From Endophytic Fungus of Sporothrix sp Against Bacteria Escherichia coli and Staphylococcus aureus

Modern drugs that are developing now come from active ingredients isolated from plants that require large amounts of plants. The development of new drugs from endophytic fungi finds problems in the amount of pure compounds produced. Therefore, further research is needed using endophytic fungi as a new antimicrobial producer. This study aims to see the ability or activity of pure compounds produced by endophytic fungi Sporothrix sp from dahlia bulbs (Dahlia variabilis). The activity test of pure compounds produced by Sporothrix sp endophytic fungus against E.coli and Staphylococcus aureus was determined by disc diffusion method. With a dose of 10.30 and 50µg/disk. In Escherichia coli bacteria doses of 10 and 50µg/disk gave a significant inhibitory effect on isolated pure compounds compared to the positive control of ciprofloxacin, which was characterized by a statistically significant test (p <0.05). In contrast to Staphylococcus aureus there was no significant difference in dosage of both 10.30 and 50 µg/disk doses. Determination of pure compounds was carried out by HPLC and Infra Red Spectrofotometric